WebThe biotin coverage of the functionalized spheres is assayed by titometric spectrophotometry at 500nm using 2-(4- hydroxyphenylazo)benzoic acid (HABA, Thermo Scientific; product number 28010, lot number BCBV7265), a fluorescent dye that binds to avidin and is displaced when avidin binds to biotin12. WebHABA/Avidin Solution Add 10 mg of avidin and 600 µl of the HABA Solution to 19.4 ml of PBS. Store solution at 4°C for up to 2 weeks. C. Procedure (Cuvette Format) 1. Pipette 900 µl of HABA/Avidin Solution into a 1 ml cuvette and measure the absorbance at 500 nm. The A500 of this solution should be 0.9-1.3. Record the value as A500 HABA ...
Supplementary Information Nanocomposites Cytosolic …
WebHABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins. Supplier: Thermo … WebThermo Scientific Pierce Protein Assay Product Information . Current versions of this and other Tech Tips are available from the Technical Resources portion of our web site. Current product instructions and a complete listing of products are also available. 23225, 23227 BCA Protein Assay Kit, working range 20-2000 g/ml cand visezi paianjen albastru
EZ-Link PFP-Biotin - Pierce
WebTranslations in context of "THERMAL" in english-indonesian. HERE are many translated example sentences containing "THERMAL" - english-indonesian translations and search engine for english translations. WebSupplier: Thermo Scientific. Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation … WebThermo Scientific™ HABA (4'-hydroxyazobenzene-2-carboxylic acid) A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins. Supplier: Thermo Scientific™ 28010 Catalog No. PI28010 $186.90 / Pack of 1 Qty Check Availability Add to cart Description can dvi do 4k