2024 Hoechst 33342 molecular probes

Hoechst 33342 molecular probes

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August undefined, 2024

NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has … NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has …

Binding of Hoechst with nucleic acids using fluorescence spectroscopy

NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … rock and roll lube

Invitrogen™ Höchst 33342, Trihydrochlorid, Trihydrat - Fisher Sci

NettetBisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several related chemical compounds are used for similar purposes and are … Nettet4. nov. 2016 · The influence of ABC transporters on the fluorescence of probes in SP and MP cells. FACS plots and mean fluorescent intensity (MFI) of sorted C6 SP (Hoechst 33342-effluxing) and MP (Hoechst 33342-retaining) cells after staining with CellROX (a, b), MTG (c, d) and JC-1 (e-g) in the presence or absence of verapamil.Fluorescence of … NettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). rock and roll lubrication

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Requirement of ABC transporter inhibition and Hoechst 33342 …

NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear … Oligos, Primers, Probes and Genes; TaqMan Real-Time PCR Assays; … NettetHoechst 33342 est un contre-colorant nucléaire populaire, perméant aux cellules, qui émet une fluorescence bleue en cas de liaison à l’ADNdb. Ce colorant est souvent utilisé … rock and roll lookNettetHoechst 33342 contains additional ethyl group. This group renders Hoechst 33342 a bit more hydrophobic than Hoechst 33258. Both dyes have the absorption band at 350 nm and the emission band in the “blue” region. They are non-intercalating fluorescent probes for non-covalent labeling of DNA. rock and roll lullaby chords

"NettetHoechst 33342 is perhaps the most ubiquitous nuclear fluorescence stain for live and fixed cells and tissue sections. The cell permeable stain binds preferentially to AT regions of DNA. It is excited by UV light and emits blue fluorescence at 460nm when bound to DNA. Ready-to-use liquid formulation of high-purity Hoechst 33342 " - Hoechst 33342 molecular probes

Hoechst 33342 molecular probes

Hoechst Stains - University of Illinois Urbana-Champaign

NettetbisBenzimide H 33342 trihydrochloride Synonym(s) : 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1 H -benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, … Nettet(Molecular Probes: C-1430). Hoechst 33342, a bisbenzimide dye, is a cell membrane permeant, minor groove-binding DNA stain that fluoresce bright blue upon binding to DNA. The dye is quite soluble in water and relatively non-toxic (Molecular Probes: H-1399, H-3570). Hoechst 33342 stains both, live and dead cells. Material and Methods Cell Culture

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NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell … NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from ... Molecular Probes, Inc. Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly

NettetHoechst 33342 25 mg. Plus de 40 ans d’expérience à votre ... Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells ... Product Type: Chemical. Molecular Formula: C27H28N6O . 3HCl . xH2O. Molecular Weight: 561.93. CAS: 23491-52-3. Source / Host: Synthetic . Purity: >98 ... Nettet19. jun. 2014 · Single molecule localization microscopy of mitotic chromosomes stained with Hoechst 33342. Comparison of (A) the widefield image (raw data, collected in 450‒490 nm range) and (B) the localization image of chromatin assessed by application of low intensity 405 nm together with high intensity 491 nm light shows the superiority of …

NettetHöchst 33342 ist eine beliebte zelldurchlässige nukleäre Gegenfärbung mit einer blauen Fluoreszenz bei Anbindung an dsDNA. Dieses Färbemittel wird häufig zur … NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed …

NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue™ Live ReadyProbes™ Reagent we …

NettetI dag · As shown in Fig. S4, the magnetic collected groups of Hoechst pre-stained S. aureus incubated with MDV 1 and MDV 2 showed bright blue fluorescent dots while the group of Hoechst-stained S. aureus incubated with MD 1 and MD 2 (without Van) only showed black background, which could ascribe to recognition of Van molecules … rock and roll lou reed lyricsNettet23. sep. 2024 · Microscopy was performed on a Carl Zeiss Axiovert 200 Μ with a Plan-Apochromat 63×/1.4NA oil lens and wavelength filter sets for detection of Hoechst 33342 (450 nm), Alexa Fluor 488 (520 nm) and Alex Fluor 555 (568 nm) The images were acquired using the high sensitivity digital camera AxioCam HRc for multicolor … rock and roll lullaby songNettetHoechst 33342 was also used to stain the cellular nuclei. As shown in Figure 2, in Ir-treated group, ... Smith RA, Hartley RC, Murphy MP. Mitochondria-targeted small molecule therapeutics and probes. Antioxid Redox Signal. 2011;15(12):3021–3038. 33. rock and roll lullaby traduçãoNettetBiotium offers both Hoechst 33342 and Hoechst 33258, ... Note: DAPI or Hoechst can be combined with other fluorescent probes. ... Molecular formula: C 27 H 31 Cl 3 N 6 O; CAS number: 23491-52-3; Recommended staining concentration: 1 ug/mL; Hoechst 33342, trihydrochloride trihydrate. rock and roll lullaby letraNettetDescription. Hoechst 33342 is perhaps the most ubiquitous nuclear fluorescence stain for live and fixed cells and tissue sections. The cell permeable stain binds preferentially to AT regions of DNA. It is excited by UV light and emits blue fluorescence at 460nm when bound to DNA. Ready-to-use liquid formulation of high-purity Hoechst 33342. rock and roll lounge long beach caNettetAdd 10 μ lof Hoechst 33342 to each 1 m of the cell suspension and mix thoroughly. Incubate the cells at 37°C for 5-15 minutes. 4. Centrifuge the cells at 1,000 rpm for five minutes at 4°C and discard the supernatant. 5. Resuspend cells in 1000 µl of 1X buffer A. 6. Add 5 μ lof PI to each 1 m of cell suspension and mix thoroughly. rock and roll lullaby - b.j. thomasNettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the … rock and roll lunch box