2024 Pin1 activity assay kit

Pin1 activity assay kit

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August undefined, 2024

WebApr 8, 2024 · Liu et al. reported that an IRAK1-PIN1 axis enhances intrinsic tumor resistance to radiation therapy in p53 ... DNA damage was detected with a comet assay kit (KeyGEN, Nanjing, China) under alkaline conditions. ... s Luciferase reporter assay for detecting the activity of wild-type or mutant IRAK1 promoters in U251 cells which were transfected ... WebJul 8, 2024 · The interaction between Pin1 and HBx was first confirmed using Ni 2+-NTA pull-down assay. His 6-tagged Pin1 ... Pin1 can enhance the transcriptional activity ... Cell Counting Kit-8 (CCK-8) assays ...

PIN1 - an overview ScienceDirect Topics

WebAssay Type Time Activity Description Data Sources; HCA2: 2.5 μM: Function Assay-Inhibits p38 mediated MK2 activation: 17964780: Hela-Function Assay-Inhibits Tat-induced HIV1 LTR transactivation in human HeLa cells with IC50 of 0.1 μM: 18926711: hESCs: 5 μM: Growth Inhibition Assay-Induces cardiomyogenic activity assessed as cell growth: … WebThe ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme classification of Peptidylprolyl isomerase. The protein encoded by PIN1 has a predicted amino acid … tauber miami

PIN1 Kits Biocompare

WebThe SensoLyte® Green Pin1 Assay Kit uses a fluorogenic substrate pretreated to convert into cis isoform. Pin1 changes this substrate into the trans conformation that is readily cleaved to generate fluorescent signal. Fluorescence is then monitored at … WebFeb 13, 2024 · SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric - 1 kit $607.00 Check your price Cat.Number : AS-72240 Availability : In … WebApr 27, 2015 · Pin1 (Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl isomerase, PPIase) is an enzyme that isomerizes phospho/Threonine-Proline motifs. Upon binding (to various target proteins) it can function as a protein regulator as it plays a role … 7度演劇

SensoLyte Green Pin1 Activity Assay Kit Fluorimetric*

WebPin1 Activity Assays. Pin1 activity was measured as previously described , with a few modifications. Briefly, for analysis of Pin1 inhibitors, SN were pretreated for 15 min at 37°C with drugs before lysates were prepared by addition of Triton X-100 to a final concentration of 1%, followed by three freeze-thaw cycles and 20 min incubation on ice. WebPIN1 is expressed in the protoderm, its polarity facing the cotyledon initiation sites, and in the vasculature, where polarity is facing downwards ( Benkova et al., 2003 ). The cotyledon initiation sites are marked by convergence of PIN1 in the adjacent membranes of … 7巧板拼图WebThis high-throughput assay system is a powerful tool for the discovery of peptidyl- ... ning time to monitor PPIase activity on one 96-well microplate is about 30 min,10 which is problematic for high-throughput screening (HTS). ... kit, HBS-EP buffer, and anti-GST antibody were obtained from GE Healthcare Bio-Sciences (Tokyo, Japan). ... tauber metall

"WebPeptidyl-prolyl isomerase (Pin1) (1) Peroxidase (3) Phosphatase (9) Phosphate (1) Plasmin (2) Plasminogen activator (2) Protease (2) Protein (1) Renin (4) Sirtuin (SIRT) (4) Sortase A (1) ... SensoLyte® 520 ADAM10 Activity Assay Kit Fluorimetric - 1 kit Cat.Number : AS-72226; Quick view SensoLyte® 520 ADAMTS13 Activity Assay Kit Fluorimetric ... " - Pin1 activity assay kit

Pin1 activity assay kit

Pin1 Functional PPIase Assay Drug Discovery Selcia

WebAug 29, 2024 · Pin1 is the only known peptidyl-prolyl cis–trans isomerase (PPIase) that specifically recognizes and isomerizes the phosphorylated Serine/Threonine-Proline (pSer/Thr-Pro) motif. The Pin1 ... WebNov 28, 2014 · Luciferase activity was determined using a luciferase assay kit (Promega) according to the manufacturer’s instructions. The relative luciferase activity was normalized against the β-galactosidase activity. ... To further understand how Pin1 is down-regulated by p53 during ER stress, we examined the effect of p53 on Pin1 promoter activity.

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WebAssay Kits; PIN1 Kits; PIN1 Kits. PIN1 Kits. Products (88) Write a Review; Company View; Product View; Your search returned 88 Assay Kit across 21 suppliers. Showing 21 of 21 suppliers (88 products total) > >> Select All. Select up to 5 products from below to … WebPIN1. The human parvulin PIN1 (EC 5.2.1.8, 163aa) is a nuclear protein implicated in the regulation of mitosis through interaction with CDC25 and polo-like kinase-1. From: The Dictionary of Cell & Molecular Biology (Fifth Edition), 2013. View all Topics. Download as …

Web第一部分、Pin1基因敲减对SH-SY5Y神经细胞氧反常损伤的影响目的：观察Pin1在不同时限SH-SY5Y神经细胞氧反常损伤中的表达变化，通过Pin1基因敲减，研究Pin1对细胞凋亡的影响及在线粒体凋亡通路中的作用机制，探讨Pin1在SH-SY5Y神经细胞氧反常损伤中的功能。方法：（1）Pin1蛋白的细胞内定位 ... WebThe SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Pin1 changes substrate into trans conformation that is readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. Increase in fluorescence intensity is directly proportional to the Pin1 activity. Order #:

WebHuman PIN1 ELISA Kit (HUFI02016) from Assay Genie is a high quality, simple protocol ELISA kit to measure PIN1 in serum, plasma & cell lysate samples Toggle menu Select Currency: EUR WebThe ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme classification of Peptidylprolyl isomerase. The protein encoded by PIN1 has a predicted amino acid length of 163, a mass of 18.2 kDa, and a cytoplasmic and nuclear subcellular localization.

WebSep 17, 2024 · The inhibitory activities of our Pin1 inhibitors, H-77, H-175, H-363, H-371 and H-596, have been confirmed to inhibit Pin1 enzyme activity in an in vitro PPIase assay using recombinant Pin1 ... taubermündungWebSensoLyte® Green PIN1 Activity Assay Kit Fluorimetric View all assay kits Protease activity assay kits for cancer research Cancer pathogenesis is closely associated with the activity of proteases. Accordingly, testing protease inhibitors is routinely investigated as … tauber mcdonaldsWebIn vitro PIN1 inhibition activity. Compounds identified by virtual screening were purchased from Pharmeks (with a purity >92%, as stated by the supplier) and tested for PIN1 inhibitory activity using the in vitro fluorescent SensoLyte Green Pin1 Assay Kit (AS-72240; AnaSpec, Fremont, CA, USA). Fluorescent values were recorded with an Infinite ... 7 幾公分WebMouse Pin1 ELISA Kit (ab209879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pin1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Pin1 with 11.2 … 7幅WebJun 2, 2014 · Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay. 1. Prepare 180ul assay buffer into a suitable container: 133mM Tris-HCl, pH8.0, 5.5nM chymotrypsin, and 0.5ug of Pin1 recombinant protein. tauber mundwinkelWebSelect your country ... 7度穿什么衣服WebDec 1, 2015 · Knockdown of Pin1 inhibited alcohol-induced cardiomyocyte apoptosis. (a) Pin1 protein expression measured by western blotting after Pin1 siRNA transfection. ((b)–(d)) Cell viability assay (b), caspase-9 and caspase-3 activity assays (c), and TUNEL staining (d) in Pin1-knockdown cardiomyocytes treated or untreated with alcohol (200 … taubermobil